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Increased serum levels of substance P in depressed patients
In the brain, the neurokinin (NK) peptide, substance P (SP), is co-localized with serotonin and noradrenaline, both of which are involved in the pathophysiology of depression. Animal studies have shown a decrease in SP content in certain brain regions after chronic antidepressant treatment and an increased serotonergic transmission following blockade of NK1 receptors. Clinically, the NK1 receptor antagonist, MK-869, has been shown to have antidepressant activity. Although a second study did not replicate this. Measurement of SP levels in cerebro-spinal fluid of depressed patients and control subjects are equivocal, and data on the density of NK1 receptors postmortem are rare. To further investigate the possible link between SP and depression, a recent study measured serum SP levels in depressed patients before and after antidepressant treatments.
Twenty-three patients diagnosed with major depression and thirty three healthy control subjects were enrolled in the study. The SP serum levels were measured, using an enzyme immunoassay, in patients at baseline (before treatment) and after 2 and 4 weeks of antidepressant therapy (mirtazapine, paroxetine or nonselective classical antidepressants in combination with either lithium or benzodiazepines). SP levels in controls were determined at baseline and after 4 weeks.
In healthy control subjects, the SP serum levels were relatively constant over the 4 weeks of the study. SP levels in drug-free, depressed patients were significantly higher than in controls. There was no change in mean SP levels between baseline and 4-week treatment with antidepressants. When individual SP levels were considered, however, 37% of them were decreased by 15% to 50%, a change which correlated with the reduction of depression severity.
The fact that serum SP levels are enhanced in some patients with major depression suggests that their central neuropeptides may also be altered and that these subjects might belong to a subgroup. Although preliminary, these findings may contribute to better understand the role of neuropeptides in depression and lead to the development of new antidepressants.
Biol Psychiatry 53: 538-542, 2003

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