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Reduced brain trapping of a-[11C]methyl-L-tryptophan, a proxy measure for serotonin synthesis, in depressed patients
The serotonin hypothesis of depression supposes a reduced availability of serotonin (5-hydroxytryptamine, 5-HT) in certain brain areas involved in the regulation of mood. Impairment of serotonin neurotransmission in patients with major depression could result from reduced availability of the precursor L-tryptophan (Trp) for serotonin synthesis, reduced metabolism and/or release, altered reuptake, receptor function and transduction. Reduction of 5-HT2 and 5-HT1A receptor binding, and 5-HT transporter have been observed in the brain of depressed patients. The object of a study was to investigate serotonin synthesis in the brain of patients with major depression, using a-[11C]methyl-L-tryptophan, a synthetic analogue of Trp, as an index of serotonin synthesis since it can be related to the metabolic conversion of Trp into serotonin.
Seventeen medication-free outpatients with major depression were compared to 17 controls. The rate of the trapping of
a-[11C]methyl-L-tryptophan (K*) was measured in different brain regions using the a-[11C]methyl-L-tryptophan/positron emission tomography (PET) method.
Normalized K* values were reduced bilaterally in the anterior cingulate cortex in women with depression, in the left side of the anterior cingulate cortex in depressed men, and in the left mesial temporal cortex in male and female patients with depression. Volumetric analysis of the subdivisions of the cingulate cortex indicated that the reduction of normalized K* in depressed patients was not due to a reduction in gray matter areas.
Since anterior cingulate cortex is thought to play a role in mood regulation, the reduction of normalized K* in some parts of the limbic and paralimbic cortices may be involved in the biochemical changes linked to major depression. This study thus provides additional evidence to support a serotonergic dysfunction associated with major depression.
Rosa-Neto P, Diksic M, Okazawa H, Leyton M, Ghadirian N, Mzengeza S, Nakai A, Debonnel G, Blier P, Benkelfat C. Measurement of brain regional {alpha}-[11C]methyl-L-tryptophan trapping as a measure of serotonin synthesis in medication-free patients with major depression. Arch Gen Psychiatry 61: 556-563, 2004.

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